Found 50 publications. Showing page 2 of 3:
2021
DNA damage and repair activity are often assessed in blood samples from humans in different types of molecular epidemiology studies. However, it is not always feasible to analyse the s#38les on the day of collection without any type of storage. For instance, certain studies use repeated sampling of cells from the same subject or samples from different subjects collected at different time-points, and it is desirable to analyse all these samples in the same comet assay experiment. In addition, flawless comet assay analyses on frozen samples opens up for the possibility of using this technique on biobank material. In this article we discuss the use of cryopreserved peripheral blood mononuclear cells (PBMCs), buffy coat (BC) and whole blood (WB) for analysis of DNA damage and repair using the comet assay. The published literature and the authors’ experiences indicate that various types of blood samples can be cryopreserved with only minor effect on the basal level of DNA damage. There is evidence to suggest that WB and PBMCs can be cryopreserved for several years without much effect on the level of DNA damage. However, care should be taken when cryopreserving WB and BCs. It is possible to use either fresh or frozen samples of blood cells, but results from fresh and frozen cells should not be used in the same dataset. The article outlines detailed protocols for the cryopreservation of PBMCs, BCs and WB samples.
Oxford University Press
2021
2020
The high persistence of PFAS is sufficient for their management as a chemical class
Royal Society of Chemistry (RSC)
2020
2020
2020
2020
2020
2020
2020
The way forward for assessing the human health safety of cosmetics in the EU - Workshop proceedings
Elsevier
2020
2019
2019
2019
The concept of essential use for determining when uses of PFASs can be phased out
Royal Society of Chemistry (RSC)
2019
2019
2019
2019
This paper presents a comprehensive review of European Union (EU) legislation addressing the safety of chemical substances, and possibilities within each piece of legislation for applying grouping and read-across approaches for the assessment of nanomaterials (NMs). Hence, this
review considers both the overarching regulation of chemical substances under REACH (Regulation (EC) No 1907/2006 on registration, evaluation, authorization, and restriction of chemicals) and CLP (Regulation (EC) No 1272/2008 on classification, labeling and packaging of substances and mixtures) and the sector-specific pieces of legislation for cosmetic, plant protection and biocidal products, and legislation addressing food, novel food, and food contact materials. The relevant supporting documents (e.g. guidance documents) regarding each piece of legislation were identified and reviewed, considering the relevant technical and scientific literature. Prospective regulatory needs for implementing grouping in the assessment of NMs were identi-
fied, and the question whether each particular piece of legislation permits the use of grouping and read-across to address information gaps was answered.
Informa Healthcare
2019
Polychlorinated biphenyls (PCBs) can be used as chemical sentinels for the assessment of anthropogenic influences on Arctic environmental change. We present an overview of studies on PCBs in the Arctic and combine these with the findings from ArcRisk—a major European Union-funded project aimed at examining the effects of climate change on the transport of contaminants to and their behaviour of in the Arctic—to provide a case study on the behaviour and impact of PCBs over time in the Arctic. PCBs in the Arctic have shown declining trends in the environment over the last few decades. Atmospheric long-range transport from secondary and primary sources is the major input of PCBs to the Arctic region. Modelling of the atmospheric PCB composition and behaviour showed some increases in environmental concentrations in a warmerArctic, but the general decline in
PCB levels is still the most prominent feature. ‘Within-Arctic’ processing of PCBs will be affected by climate change-related processes such as changing wet deposition. These in turn will influence biological exposure and uptake of PCBs. The pan-Arctic rivers draining large Arctic/sub-Arctic catchments provide a significant source of PCBs to the Arctic Ocean, although changes in hydrology/sediment transport combined with a changing marine environment remain areas of uncertainty with regard to PCB fate. Indirect effects of climate change on human exposure, such as a changing diet will influence and possibly reduce PCB
exposure for indigenous peoples. Body burdens of PCBs have declined since the 1980s and are predicted to decline further.
2018
2018
Exposure to airborne fine particulate matter (PM2.5) carries substantial health risks, particularly for younger children (0–10 years). Epidemiological evidence indicates that children are more susceptible to PM health effects than adults. We conducted a literature review to obtain an overview of existing knowledge regarding the correlation of exposure to short- and long-term PM concentrations with respiratory symptoms and disease in children. A collection of scientific papers and topical reviews were selected in cooperation with two experienced paediatricians. The literature review was performed using the keywords “air pollution”, “particulate matter”, “children’s health” and “respiratory” from 1950 to 2016, searching the databases of Scopus, Google Scholar, Web of Science, and PubMed. The search provided 45,191 studies for consideration. Following the application of eligibility criteria and experts’ best judgment to titles and abstracts, 28 independent studies were deemed relevant for further detailed review and knowledge extraction. The results showed that most studies focused mainly on the effect of short-term exposure in children, and the reported associations were relatively homogeneous amongst the studies. Most of the respiratory diseases observed in outdoor studies were related to changes in lung function and exacerbation of asthma symptoms. Allergic reactions were frequently reported in indoor studies. Asthma exacerbation, severe respiratory symptoms and moderate airway obstruction on spirometry were also observed in children due to various sources of indoor pollution in households and schools. Mixed indoor and outdoor studies indicate frequent occurrence of wheezing and deterioration of lung function. There is good evidence of the adverse effect of short-term exposure to PM on children’s respiratory health. In terms of long-term exposure, fine particles (PM0.1–PM2.5) represent a higher risk factor than coarse particles (PM2.5–PM10). Additional research is required to better understand the heterogeneous sources and the association of PM and adverse children’s health outcomes. We recommend long-term cooperation between air quality specialists, paediatricians, epidemiologists, and parents in order to improve the knowledge of PM effects on young children’s respiratory health.
MDPI
2018
2018
The comet assay applied to cells of the eye
Oxford University Press
2018